Manchester United face Danish side Midtjylland in the last 32 of the Europa League after dropping out of the Champions League.
Liverpool boss Jurgen Klopp will make a return to Germany as the Reds play Augsburg.
Tottenham Hotspur have been drawn against Serie A title chasers Fiorentina for the second consecutive season at this stage.
Gary Neville’s Valencia have been paired with Rapid Vienna.
The first legs are on Thursday, 18 February, with the return ties a week later.
All three English clubs will be at home for the second leg because they were seeded.
Spurs and Liverpool qualified after winning Group J and B respectively, while United finished third in Champions League Group B.
Draw in full
Valencia v Rapid Vienna
Fiorentina v Tottenham Hotspur
Borussia Dortmund v FC Porto
Fenerbahce v Lokomotiv Moscow
Anderlecht v Olympiakos
Midtjylland v Manchester United
Augsburg v Liverpool
Sparta Prague v Krasnodar
Galatasaray v Lazio
Sion v Braga
Shakhtar Donetsk v Schalke
Marseille v Athletic Bilbao
Sevilla v Molde
Sporting Lisbon v Bayer Leverkusen
Villarreal v Napoli
Saint-Etienne v Basel
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